Habilitationsvortrag Physik: Magnetic tweezers to study gene machines at the single-molecule level

Oct 17
17. October 2018 12:00 - 13:00
Hörsaal H, Staudtstr. 5, 91058 Erlangen

Bitte beachten Sie, dass der Vortrag nun erst um 12:00 Uhr in Hörsaal H stattfindet!

Einladung zum Habilitationsvortrag von Herrn David Dulin, Interdisziplinäres Zentrum für Klinische Forschung, FAU:
„Magnetic tweezers to study gene machines at the single-molecule level “

Every organism contains a piece of genetic information, in the form of either DNA, e.g. for any living organism, or RNA, e.g. for RNA virus. During the life cycle of an organism, nanoscopic molecular machines called enzymes express and maintain the genome. Enzymes are characterized by an underlying instability, where multiple free energy states separated by low activation energy, i.e. typically few kBT (kB being the Boltzmann constant) interconvert rapidly through complex kinetic pathways. Therefore, in the wet and hot environment of the cell, enzymatic activity is noisy, asynchronous and heterogeneous. To characterize precisely a given enzyme without averaging all the different kinetic states, it is therefore important to observe each enzyme individually, i.e. the single molecule level, and at the spatiotemporal resolution that matters, i.e. ~1 nm at 10-100 ms. Several techniques have been developed in the last thirty years to manipulate and pull on single biomolecules, e.g. optical tweezers, magnetic tweezers and atomic force microscopy. Magnetic tweezers are a torque and force spectroscopy technique that have been developed to study the mechanical properties of nucleic acids, i.e. DNA and RNA, and protein-nucleic acids interactions at the single-molecule level. During the presentation, I will describe the technique and how magnetic tweezers are applied in my lab to study several gene machines.

(Vortrag auf Englisch)